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Rat tissue sections were pretreated for 15 min with HIER at 95 C in ER2 (Leica) followed by a 15-min protease treatment and then hybridized with rat-specific probes to detect mRNA transcripts for CALCR (477798), PRLH (1301288-C2) and GLP1R (315228-C3

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doi: 10.2967/jnumed.120.261594 76 WangBPeiJXuSLiuJYuJ

Rather than protecting the peptide through the stomach, this formulation aims for sublingual and buccal absorption